OhMyCalc

Cell Viability Calculator

Calculate cell viability percentage and viable cell concentration from trypan blue exclusion assay data.

How to Use the Cell Viability Calculator

  1. Enter the count of live (unstained) cells.
  2. Enter the count of dead (stained) cells.
  3. Enter the dilution factor.
  4. Enter the number of squares counted.

使用场景

公式

Viability = live / (live + dead) × 100%; Conc = (live / squares) × DF × 10⁴

常见问题

How accurate is this calculator?
Results are based on standard scientific formulas and are suitable for preliminary estimates.
What units are used?
SI units (metres, kg, seconds) are used unless otherwise noted.
Is it free?
Yes, all calculators are completely free.