Cell Viability Calculator
Calculate cell viability percentage and viable cell concentration from trypan blue exclusion assay data.
How to Use the Cell Viability Calculator
- Enter the count of live (unstained) cells.
- Enter the count of dead (stained) cells.
- Enter the dilution factor.
- Enter the number of squares counted.
Use Cases
- •Cell culture quality control
- •Drug cytotoxicity testing
- •Research lab cell counting
Formula
Viability = live / (live + dead) × 100%; Conc = (live / squares) × DF × 10⁴
Frequently Asked Questions
How accurate is this calculator?
Results are based on standard scientific formulas and are suitable for preliminary estimates.
What units are used?
SI units (metres, kg, seconds) are used unless otherwise noted.
Is it free?
Yes, all calculators are completely free.