Enzyme Kinetics Calculator (Michaelis-Menten)
Calculate reaction velocity using the Michaelis-Menten equation: v = (Vmax × [S]) / (Km + [S]).
How to Use the Enzyme Kinetics Calculator
- Enter the maximum velocity (Vmax) in your chosen units.
- Enter the Michaelis constant (Km) in the same concentration units as substrate.
- Enter the current substrate concentration [S].
- Click Calculate to see the reaction velocity and percent saturation.
Casos de Uso
- •Modeling enzyme behavior at varying substrate concentrations.
- •Comparing enzyme efficiency (Vmax/Km) across mutants or conditions.
- •Predicting metabolic flux in biochemical pathway modeling.
Fórmula
Michaelis-Menten: v = (Vmax × [S]) / (Km + [S]). When [S] = Km, v = Vmax/2.
Perguntas Frequentes
What is Km?
Km (Michaelis constant) is the substrate concentration at which the reaction velocity is half of Vmax. A low Km indicates high enzyme affinity for the substrate.
What is Vmax?
Vmax is the maximum reaction velocity achieved when the enzyme is fully saturated with substrate. It depends on total enzyme concentration and the catalytic rate constant (kcat).
Disclaimer
For educational and research purposes only. The Michaelis-Menten model assumes steady-state conditions, single substrate, and no product inhibition. Real enzymes may show more complex kinetics.