Beer-Lambert Law Calculator
Calculate absorbance, concentration, molar absorptivity, or path length using the Beer-Lambert law: A = ε × c × l.
How to Use the Beer-Lambert Calculator
- Enter any three of the four values: absorbance, molar absorptivity, concentration, path length.
- Leave the field you want to calculate empty or at zero.
- Click Calculate — the tool will solve for all computable unknowns.
Casi d'Uso
- •Calculating unknown concentrations from spectrophotometer readings.
- •Verifying molar absorptivity values for new chromophores.
- •Teaching analytical chemistry and spectrophotometry concepts.
Formula
Beer-Lambert Law: A = ε × c × l. A = absorbance (dimensionless), ε = molar absorptivity (M⁻¹cm⁻¹), c = concentration (M), l = path length (cm).
Domande Frequenti
What is the Beer-Lambert law?
The Beer-Lambert law states that absorbance is proportional to the concentration of a solution and the path length of light through it. It is fundamental to spectrophotometry and is valid at low concentrations where interactions between molecules are negligible.
At what absorbance range is the Beer-Lambert law linear?
Linearity is typically maintained at absorbance values between 0.1 and 1.0. Above A = 1.5 the relationship often deviates due to stray light, detector non-linearity, and solute-solute interactions.
Disclaimer
For educational and laboratory purposes only. The law assumes monochromatic light, dilute solutions, and no scattering. Deviations occur with turbid samples or high concentrations.