Hemocytometer Cell Count Calculator
Calculate cell concentration in cells/mL from hemocytometer counts, number of squares counted, and dilution factor.
How to Use the Hemocytometer Calculator
- Count cells in your chosen squares under the microscope.
- Enter the total number of cells counted.
- Enter the number of large squares counted (usually 4–5).
- Enter the dilution factor applied to the sample.
- Click Calculate to get cells/mL and cells/µL.
Use Cases
- •Counting viable and total cells for cell culture seeding.
- •Determining yeast cell density for fermentation inoculation.
- •Counting blood cells or sperm cells in clinical and research labs.
Formula
Formula: cells/mL = (cell count × dilution factor) / (squares counted × 0.0001). Each large Neubauer square has a volume of 0.1 µL = 0.0001 mL.
Frequently Asked Questions
What is a hemocytometer?
A hemocytometer is a counting chamber used with a microscope to determine the number of cells in a liquid sample. The standard Neubauer chamber has a grid of 9 large squares, each with a volume of 0.1 µL.
How many squares should I count?
Typically count 4–5 large corner squares plus the center square for best accuracy. Count cells touching the top and left boundaries but not the bottom and right boundaries.
Disclaimer
For educational and laboratory purposes only. Cell counting accuracy depends on proper technique, dilution, and staining. Always perform replicate counts.