OhMyCalc

Chromatography Rf Calculator

Calculate Rf value from substance migration distance and solvent front distance in TLC or paper chromatography.

How to Use the Rf Calculator

  1. Measure the distance the substance traveled from the baseline.
  2. Measure the distance the solvent front traveled.
  3. Enter both values and click Calculate.

Use Cases

Formula

Formula: Rf = Distance traveled by substance / Distance traveled by solvent front.

Frequently Asked Questions

What is Rf value?
The Rf (retardation factor) is a dimensionless value between 0 and 1 that indicates how far a substance travels relative to the solvent front in chromatography.
What Rf value indicates a non-polar compound?
In normal-phase TLC with non-polar solvents, non-polar compounds have high Rf values (>0.7). In reverse-phase, the opposite applies.
Can Rf be greater than 1?
No. Rf values range from 0 (no movement) to less than 1 (less than solvent front). Values greater than 1 indicate measurement error.