Fungal Spore Count Calculator
Compute fungal spore density per millilitre from a hemocytometer count and dilution factor using the simplified formula C = count × 10⁴ × dilution.
How to Use the Fungal Spore Count Calculator
- Count spores in the Neubauer chamber.
- Enter the total spores counted.
- Enter the dilution factor applied before counting.
- Click Calculate.
Casi d'Uso
- •Inoculum preparation.
- •Fungal growth experiments.
- •Teaching mycology lab technique.
Formula
C (spores/mL) = count × 10⁴ × dilution. The 10⁴ factor assumes a standard Neubauer chamber with 0.1 mm³ volume per large square.
Domande Frequenti
Which chamber does the 10⁴ assume?
A standard Neubauer hemocytometer: each large square is 1 mm × 1 mm × 0.1 mm = 10⁻⁴ mL. So total spores per mL = spores counted × 10⁴ × dilution.
How many squares should I count?
Count at least 4 large squares and average. For precision below 5% coefficient of variation, count 5 or more.